Fluorescence detection

Filter Sets for qPCR and Digital PCR Systems

Multi-channel PCR systems depend on clean separation between excitation and emission light. Lumalyx structures filter-set requirements around channel wavelength, FWHM, blocking depth, AOI and detector response before RFQ.

FAM / HEX / ROX / Cy5Excitation filtersEmission filtersDichroic mirrorsCrosstalk control
Example spectral response curve for qPCR and Digital PCR Filter Sets
RFQ-ready context

Translate spectral requirements into manufacturable fields.

This page is structured for engineers and technical buyers: application language first, then optical parameters, risk notes and an RFQ path.

Typical componentsExcitation bandpass, emission bandpass, dichroic mirror
Key parametersCWL, FWHM, T%, OD, blocking range, AOI, size
Design riskChannel bleed-through, background fluorescence, low signal margin
RFQ pathUpload dye spectra or existing filter curves, then generate a channel package

Need a custom optical set?

Upload an existing specification, spectrum, drawing or channel list. Lumalyx packages the submitted fields into a technical request for engineering review.

Generate RFQ package
Application review

Application review checkpoints.

Lumalyx keeps application pages focused on engineering inputs rather than outcome claims. Each request is reviewed as a signal-path problem before component selection.

Signal definition

Clarify source, detector, target band, adjacent channels and background risks.

Component match

Map the requirement to filters, dichroic mirrors, beamsplitters, lenses or coated parts.

RFQ package

Convert wavelength, geometry, coating and quantity fields into a structured review request.

Fields to prepare before review.

  • Working wavelength or channel list.
  • CWL, FWHM, transmission and blocking target where known.
  • Angle of incidence, substrate, size and thickness.
  • Existing spectra, drawings, reference curves or supplier notes.
  • Application constraints such as packaging, environment and expected quantity.